Hossein Baharvand, Ph.D
Abdolhossein Shahverdi, Ph.D
Abdolhossein Shahverdi received his Ph.D in Anatomy from Tarbiat Modarres University in 2007. Since 1999, he is the Vice President of the Department of Research and Education, an Assistant Professor and academic member of Royan Institute.
Mohsen Abolfathi (M.Sc)
Farnoosh Attari (Ph.D)
Faezeh Moraveji (M.Sc)
Spermatogonial stem cells (SSCs) are unipotent precursor cells for sperm generation in the testis. SSCs represent an extremely low proportion (0.02%–0.03%) of the cells of the testis, but they can be isolated and propagated in vitro. More recently, it was found that these cells can be reprogrammed spontaneously to multipotent cells in vitro and have the capability to differentiate into various differentiated cell lineages. In fact, these multipotent cells can act like embryonic stem (ES) cells in their differentiation properties and in the morphology and growth characteristics of the colonies they form in culture. Thus, they are named ES-like cells. These findings made the field more exiting, and several reports have commented on the possible uses of these SSC-derived ES-like cells, including regenerative medicine. Furthermore, these cells may provide new opportunities to study genetic diseases in various cell lineages. However, the rate of conversion of testicular cells to ES like cells is too low and our aim is to increase this rate in an efficient way.
Our group seeks to find an efficient way to culture human and mouse spermatogonial stem cells. Moreover, we are attempting to induce pluripotency in testicular cells with different reagents.
ES-like cells derived from testicular cells at day 6.
Tavakolifar F, et al. The effect of laminin and ilemma extracellular matrix on short-term cultivation of neonate mouse spermatogonial stem cells, Journal of Iranian Anatomical Sciences . 2010;8:37-48.