Cytometry and Imaging
Ehsan Janzamin (B.Sc)
Pardis Khosravani (M.Sc)
Fazel Sahraneshin Samani (M.Sc)
This lab, established in 2007, is equipped with a FACS ilemma. Flow cytometry, typically using fluorescent probes which bind to specific cell-associated molecules, allows for measurements of various phenotypic, biochemical and molecular characteristics of individual cells (or particles) suspended in a fluid stream. Since the latter part of 2010, we began to sort different types of stem cells using BD FACS Aria II.
The key aims of our facility are analyzing, sorting and imaging of numerous samples and cells needed by researchers. Moreover, to train users to obtain the best possible imaging and cytometry data for their experiments. Our cytometry facility is available for use by outside groups on a special basis.
Our mission is to provide state of the art multicolor flow cytometry services to the Research staff at RI-SCBT. Our educational program provides didactic lectures and hands-on experience with isolation, preparation and staining of all types of human and animal cells, instrument setup and acquisition, and data analysis.
A Becton-Dickinson FACS Calibur is available to provide analytic capability. The FACS Calibur has a five parameter analysis capability – forward and side scatter and three colors of fluorescence using 488 excitations. The instrument currently uses a MAC G5 computer for instrument control and data acquisition. The software running the FACS Calibur is CellQuest.
BD FACSAria Cell SorterI improvements in fluidics and optics have allowed for higher speed sorting (25,000 events/sec at 70psi using a 70um nozzle) with enhanced overall sensitivity
IX71 inverted microscope
Immunostained cells or tissues can be observed as well as precise, high quality photographs taken by the BX51 and IX71 microscopes located in the imaging room.