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Two Workshops on July 3rd and 4th PDF Print E-mail
Jul 06, 2013 at 10:02 AM
Royan Institute had held two scientific workshops on Genetic Engineering & Molecular Cloning and Gene Expression Analysis by Real-time PCR on July 3rd and 4th, 2013.
The Genetic Engineering and Molecular Cloning
workshop was carried out by the Reproductive Genetics group at Royan Institute for Reproductive Biomedicine. 14 researchers and students attended this laboratory program which provided experience and knowledge in genetic engineering and molecular cloning applications and processes.
 
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This workshop's hands-on activities were including:
  • An Introduction on gene cloning
  • Preparation of culture media
  • Cultivation of bacteria
  • Ligation Reaction
  • Transformation of recombinant plasmids
  • Preparation of bacterial competent cells
  • Colony PCR
  • Plasmid Extraction
  • Digestion of recombinant plasmid
  • Agarose and sample preparation  
  • Gel electrophoresis and data analysis
A Amiri Yekta was in charge of scientific management of this two-day workshop whose executive manager was M Totonchi.
 
 
The Gene Expression Analysis by Real-time PCR workshop was organized by molecular biology lab of stem cells and developmental biology department, Royan Institute for Stem Cell Biology and Technology.

At this theoretical-practical workshop, levels on gene expression analysis were discussed, RNA was extracted from cultured cells following cDNA synthesis and RT-PCR was done for target gene and reference gene. The application of Real-time PCR and the methods were also discussed and trainees ran a sample reaction and analyzed data with REST package. 13 researchers and students in biomedicine, veterinary science, genetics and laboratory science participated in this two-day workshop.
 
Dr H Baharvand was the scientific manager and A Fathi the executive secretary of this workshop.

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